Kk. Ohlemiller et al., Targeted mutation of the gene for cellular glutathione peroxidase (Gpx1) increases noise-induced hearing loss in mice, JARO, 1(3), 2000, pp. 243-254
Reactive oxygen species (ROS) and oxidative stress have been implicated in
cochlear injury following loud noise and ototoxins. Genetic mutations that
impair antioxidant defenses would be expected to increase cochlear injury f
ollowing acute insults and to contribute to cumulative injury that presents
as age-related hearing loss. We examined whether genetically based deficie
ncy of cellular glutathione peroxidase, a major antioxidant enzyme, increas
es noise-induced hearing loss in mice. Two-month-old "knockout" mice with a
targeted inactivating mutation of the gene coding for glutathione peroxida
se (Gpx1) and wild type controls were exposed to broadband noise for one ho
ur at 110 dB SPL. Auditory brainstem response (ABR) thresholds at test freq
uencies ranging from 5 to 40 kHz were obtained two and four weeks after exp
osure to determine the stable permanent component of the hearing loss. Depe
nding on test frequency, (compared with controls) Gpx1 knockout mice showed
up to 16 dB higher ABR thresholds prior to noise exposure, and up to 15 dB
greater noise-induced hearing loss, compared with normal control. Within t
he cochlear base, there was also a significant contribution of the knockout
to inner and outer hair cell loss, as well as nerve fiber loss. Our result
s support a link between genetic impairment of antioxidant defenses, vulner
ability of the cochlea injury, and cochlear degeneration. Such impairment p
roduces characteristics expected of some mutations associated with age-rela
ted hearing loss and offers one possible mechanism for their action.