Use of electrospray mass spectrometry for mass determination of grape (Vitis vinifera) juice pathogenesis-related proteins: A potential tool for varietal differentiation

Citation
Y. Hayasaka et al., Use of electrospray mass spectrometry for mass determination of grape (Vitis vinifera) juice pathogenesis-related proteins: A potential tool for varietal differentiation, J AGR FOOD, 49(4), 2001, pp. 1830-1839
Citations number
36
Categorie Soggetti
Agricultural Chemistry","Chemistry & Analysis
Journal title
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN journal
00218561 → ACNP
Volume
49
Issue
4
Year of publication
2001
Pages
1830 - 1839
Database
ISI
SICI code
0021-8561(200104)49:4<1830:UOEMSF>2.0.ZU;2-S
Abstract
Methods based on liquid chromatography-mass spectrometry (LC-MS) and protei n trap mass spectrometry (trap-MS) were developed to determine the compleme nt of pathogenesis-related (PR) proteins in grape juice. Trap-MS was superi or to LC-MS in terms of simplicity, rapidity, and sensitivity. Proteins wit h a wide range of masses (13-33 kDa) were found in the juices of 19 differe nt varieties of grape (Vitis vinifera) and were identified as mostly PR-5 t ype (thaumatin-like) and PR-3 type (chitinases) proteins. Although the PR p roteins in juices of grapes are highly conserved, small consistent differen ces in molecular masses were noted when otherwise identical proteins were c ompared from different varieties. These differences persisted through diffe rent harvest years and in fruits grown in different Australian locations. W ith the definition of four different masses for PR-5 proteins (range = 2123 9-21272 Dal and nine different masses of PR-3 proteins (range 25330-25631 D al and using statistical analysis, the methods developed could be used for varietal differentiation of grapes grown in several South Australian locati ons on the basis of the PR protein composition of the juice. It remains to be seen whether this technology can be extended to grapes grown worldwide a nd to wine and other fruit-derived products to assist with label integrity to the benefit of consumers.