IMMUNOHISTOCHEMICAL LOCALIZATION OF TYPE-II (AT(2)) ANGIOTENSIN RECEPTORS WITH A POLYCLONAL ANTIBODY AGAINST A PEPTIDE FROM THE C-TERMINAL TAIL

A polyclonal antibody has been prepared against a synthetic peptide de rived from the C-terminal tail of the cloned rat AT(2) angiotensin rec eptor, corresponding to amino acid residue 341-351. The antibody was o f high titer and displayed monospecific activity coward the synthetic peptide in the ELISA assay. Western blot analysis indicated that the a ntiserum recognised only a single protein band with a mean apparent mo lecular mass of 75.4 kDa in the rat adrenals. Immunohistochemical stud ies with affinity purified antibody localised immunoreactive AT(2) ang iotensin receptor in medulla cells of the adrenals. Immunoreactivity w as also observed in pyramidal tract, but no specific immunoreactivity can be detected in regions of rat brain that are known to express AT(2 ) angiotensin receptors, including inferior olive, locus coeruleus and cerebellum. (C) 1997 Elsevier Science B.V.