Induction of CD54 and CD58 expression in cultured human endothelial cells by beta-interferon with or without hyperthermia in vitro

Human endothelial cells were treated by beta -interferon with or without hy perthermia at 42 degreesC for 90 min in vitro to investigate whether these modalities were able to increase the expression of either CD54 or CD58 on t he surface of the endothelial cells. The results were that the population o f the endothelial cells expressing both CD54 and CD58 increased 4 days afte r the treatment with beta -interferon, which was independent of hyperthermi a. In contrast, the primarily isolated peripheral lymphocytes from a patien t with malignant melanoma (disease free state) or normal individuals respon ded to neither beta -interferon nor hyperthermia in terms of the expression of CD54 or CD58. These results indicate that beta -interferon may activate endothelial cells to lead to the successive activation of the other immune cells in vivo. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.