Automated purification of Borrelia burgdorferi s.l. PCR products with KingFisher (TM) magnetic particle processor prior to genome sequencing

Authors
Makinen, J Marttila, H Viljanen, MK
Citation
J. Makinen et al., Automated purification of Borrelia burgdorferi s.l. PCR products with KingFisher (TM) magnetic particle processor prior to genome sequencing, J MAGN MAGN, 225(1-2), 2001, pp. 134-137
Citations number
4
Categorie Soggetti
Apllied Physucs/Condensed Matter/Materiales Science
Journal title
JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS
ISSN journal
03048853 → ACNP
Volume
225
Issue
1-2
Year of publication
2001
Pages
134 - 137
Database
ISI
SICI code
0304-8853(200104)225:1-2<134:APOBBS>2.0.ZU;2-Z
Abstract
Borrelia burgdorferi sensu late genospecies were differentiated by PCR-base d sequencing of the borrelial flagellin gene. To evaluate the usefulness of KingFisher (TM) magnetic particle processor in PCR product purification, b orrelia PCR products were purified with KingFisher (TM) magnetic particle p rocessor prior to cycle sequencing and the quality of the sequence data rec eived was analyzed. Kingfisher was found to offer a rapid and reliable alte rnative for borrelial PCR product purification. (C) 2001 Elsevier Science B .V. All rights reserved.