Progenitor cell isolation with a high-capacity quadrupole magnetic flow sorter

Authors
Moore, LR Rodriguez, AR Williams, PS McCloskey, K Bolwell, BJ Nakamura, M Chalmers, JJ Zborowski, M
Citation
Lr. Moore et al., Progenitor cell isolation with a high-capacity quadrupole magnetic flow sorter, J MAGN MAGN, 225(1-2), 2001, pp. 277-284
Citations number
16
Categorie Soggetti
Apllied Physucs/Condensed Matter/Materiales Science
Journal title
JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS
ISSN journal
03048853 → ACNP
Volume
225
Issue
1-2
Year of publication
2001
Pages
277 - 284
Database
ISI
SICI code
0304-8853(200104)225:1-2<277:PCIWAH>2.0.ZU;2-K
Abstract
Rapid and efficient sorting is important for progenitor cell isolation. The refore, we have built and evaluated a high-speed, continuous flow, quadrupo le magnetic sorter. The cross-over behavior of non-magnetic particles, comb ined with separation theory is used to optimize performance. CD34 + progeni tor cells are separated to purities from 64-95% and a throughput of 10(7) c ells/s. (C) 2001 Elsevier Science B.V. All rights reserved.