Adhesion of immunomagnetic particles targeted to antigens and cytokine receptors on tumor cells determined by magnetophoresis

Authors
Tchikov, V Winoto-Morbach, S Kabelitz, D Kronke, M Schutze, S
Citation
V. Tchikov et al., Adhesion of immunomagnetic particles targeted to antigens and cytokine receptors on tumor cells determined by magnetophoresis, J MAGN MAGN, 225(1-2), 2001, pp. 285-293
Citations number
11
Categorie Soggetti
Apllied Physucs/Condensed Matter/Materiales Science
Journal title
JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS
ISSN journal
03048853 → ACNP
Volume
225
Issue
1-2
Year of publication
2001
Pages
285 - 293
Database
ISI
SICI code
0304-8853(200104)225:1-2<285:AOIPTT>2.0.ZU;2-1
Abstract
The adhesion of immunomagnetic particles to surfaces of target cells based on ligand-receptor interactions was evaluated by counting the number of mag netically labeled cells using magnetophoresis and analyzed by the Langmuir adsorption theory. As few as 200 tumor necrosis factor (TNF) receptor molec ules per cell could be detected with streptavidin-coated magnetic particles coupled to biotinylated TNF as ligand. (C) 2001 Elsevier Science B.V. All rights reserved.