Differential expression of p16/p21/p27 and cyclin D1/D3, and their relationships to cell proliferation, apoptosis, and tumour progression in invasiveductal carcinoma of the breast
Scc. Wong et al., Differential expression of p16/p21/p27 and cyclin D1/D3, and their relationships to cell proliferation, apoptosis, and tumour progression in invasiveductal carcinoma of the breast, J PATHOLOGY, 194(1), 2001, pp. 35-42
Citations number
28
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research Diagnosis & Treatment
In order to understand the intricate relationship of cell proliferation and
apoptosis in tumour development, proliferation markers (Ki-67 and c-myc),
apoptosis, cell-cycle inducers cyclin D1 and D3, and cell-cycle inhibitors
p16(INK4), p21(CIP1), and p27(KIP1) were evaluated in ductal breast carcino
ma. The heterogeneous nature of breast tumours provides a system by which t
he changes in cell-cycle genes can be explored under a wide range of prolif
eration and apoptotic indices. To address the above issues, immunohistochem
ical studies were conducted in 40 pairs of tumours and adjacent normal duct
al tissues. The TUNEL method was used to identify apoptotic cells. Except f
or p27/KIP1, the proliferation (Ki-67, c-myc) and the apoptotic indexes tog
ether with levels of p16/INK4a, p21/CIP1, cyclin D1, and cyclin D3, were cl
early elevated among tumour tissues, while absent in the adjacent normal ti
ssues. Spearman correlation analysis indicated strong associations among ap
optotic index, Ki-67, c-myc, and tumour grade. In addition, p21/CIP1 and cy
clin D3 were positively correlated, while p16/INK4a, p27/KIP1, and cyclin D
1 were negatively correlated with tumour grade. There was clear decoupling
between p21 and p27, as well as decoupling between cyclin D1 and cyclin D3,
in terms of their relationship to cell proliferation and apoptosis, indica
ting differential roles in tumour progression. Copyright (C) 2001 John Wile
y & Sons, Ltd.