The isolation and identification of eupafolin and hispidulin in the dried l
eaves of Eupatorium littorale (Asteraceae) are described. A method for the
detection of those flavonoids and rutin was also developed using reversed-p
hase HPLC with a photodiode array detector. Eupafolin and hispidulin were q
uantified and the concentrations found were 0.29% and 0.21%, respectively.