DNA TYPING OF THE D1S80 LOCUS USING CAPILLARY ZONE ELECTROPHORESIS

DNA typing of the D1S80 locus was carried out using capillary polyacry lamide gel zone electrophoresis (CZE). The D1S80 allelic ladder was is olated within 40 min in polyacrylamide gel columns of 50 mu m inner di ameter and 30 cm length. The PCR products and ladder marker in the inj ection port were electrophoresed and detected by UV absorption (254 nm ). The typing was determined according to migration of DNA fragments o n the electropherograms, which permits simultaneous analysis of both t he test sample polymerase chain reaction products and the ladder marke r. The two-step sample injection method applied in this study allows u s to use minute amounts of the ladder maker. This procedure provides a reliable, economical and rapid method for D1S80 typing. (C) 1997 Else vier Science Ireland Ltd.