Attempt to separate the fluorescence spectra of adrenaline and noradrenaline using chemometrics

An investigation was conducted on whether the fluorescence spectra of the v ery similar catecholamines adrenaline and noradrenaline could be separated using chemometric methods. The fluorescence landscapes (several excitation and emission spectra were measured) of two data sets with respectively 16 a nd 6 samples were measured, the smaller data set with higher resolution and i.e. precision. The samples were artificial urine (pH similar or equal to 3) spiked with the catecholamines in the concentration ranges 40-1200 nmol/ L and 5.5-18 mu mol/L, respectively. Unfold partial least squares regressio n (Unfold-PLSR) on the larger data set and parallel factor analysis (PARAFA C) of the six samples of the smaller set showed that there was no differenc e between the fluorescence landscapes of adrenaline and noradrenaline. It c an be concluded that chemometric separation of adrenaline and noradrenaline is not obtainable using this type of fluorescence measurement. Raman scatt er. which overlaps the catecholamine spectra, was shown not to have any inf luence on the models calculated. Copyright (C) 2001 John Wiley & Sons, Ltd.