H. Ishak et al., Comparative sequences of two type 1 dengue virus strains possessing different growth characteristics in vitro, MICROB IMMU, 45(4), 2001, pp. 327-331
The complete genome sequences of two dengue-1 virus strains having differen
t growth characteristics (Mochizuki and A88) were compared with other publi
shed strains. The sequence analysis indicated several unique amino acid cha
nges throughout the coding region of Mochizuki strain, mostly in envelope (
E) protein. A unique amino acid, Ile-69 for Mochizuki strain at E protein r
esulted in the loss of an Asn-67-linked glycosylation site. A Thr substitut
ion for ALa-114 at C protein and amino acid changes found in E, non-structu
ral NS3, NS4a, and NS5 proteins were unique for A88 strain. These substitut
ions might be correlated to their different growth characteristics in vitro
.