Comparative sequences of two type 1 dengue virus strains possessing different growth characteristics in vitro

Authors
Ishak, H Takegami, T Kamimura, K Funada, H
Citation
H. Ishak et al., Comparative sequences of two type 1 dengue virus strains possessing different growth characteristics in vitro, MICROB IMMU, 45(4), 2001, pp. 327-331
Citations number
31
Categorie Soggetti
Microbiology
Journal title
MICROBIOLOGY AND IMMUNOLOGY
ISSN journal
03855600 → ACNP
Volume
45
Issue
4
Year of publication
2001
Pages
327 - 331
Database
ISI
SICI code
0385-5600(2001)45:4<327:CSOTT1>2.0.ZU;2-R
Abstract
The complete genome sequences of two dengue-1 virus strains having differen t growth characteristics (Mochizuki and A88) were compared with other publi shed strains. The sequence analysis indicated several unique amino acid cha nges throughout the coding region of Mochizuki strain, mostly in envelope ( E) protein. A unique amino acid, Ile-69 for Mochizuki strain at E protein r esulted in the loss of an Asn-67-linked glycosylation site. A Thr substitut ion for ALa-114 at C protein and amino acid changes found in E, non-structu ral NS3, NS4a, and NS5 proteins were unique for A88 strain. These substitut ions might be correlated to their different growth characteristics in vitro .