A helical lid converts a sulfotransferase to a dehydratase

We report here the crystal structure of retinol dehydratase, an enzyme that catalyzes the synthesis of anhydroretinol. The enzyme is a member of the s ulfotransferase superfamily and its crystal structure reveals the insertion of a helical lid into a canonical sulfotransferase fold. Site-directed mut ations demonstrate that this inserted lid is necessary for anhydroretinol p roduction but not for sulfonation; thus, insertion of a helical lid can con vert a sulfotransferase into a dehydratase.