Effect of genistein analogs on oxygen radical production in leukocytes stimulated by unopsonized zymosan

Effects of genistein analogs on oxygen radical production have been analyze d in human neutrophils, human monocytes or murine macrophages Raw264.7 stim ulated with unopsonized zymosan by lucigenin- and luminol-enhanced chemilum inescence assays. Genistein exhibited IC50 values of 10.7 - 11.5 muM on the oxygen radical production in human neutrophils, 10.9 - 11.0 muM in human m onocytes, and 14.8 - 27.3 muM in Raw264.7 cells. Orobol, a genistein analog with an additional hydroxy group at the 3' position, exhibited IC50 values of 3.0 - 3.3 muM on the oxygen radical production in human neutrophils, 2. 8 - 3.1 muM in human monocytes, and 1.5 - 3.9 muM in Raw264.7 cells, Genist in and sophoricoside are genistein glycosides with a glucose moiety at 7 or 4' position, respectively. The genistein glycosides exhibited 23-37% inhib itory effects at 100 muM on the oxygen radical production.