PRAC: A novel small nuclear protein that is specifically expressed in human prostate and colon

Citation
Xf. Liu et al., PRAC: A novel small nuclear protein that is specifically expressed in human prostate and colon, PROSTATE, 47(2), 2001, pp. 125-131
Citations number
26
Categorie Soggetti
Urology & Nephrology","da verificare
Journal title
PROSTATE
ISSN journal
02704137 → ACNP
Volume
47
Issue
2
Year of publication
2001
Pages
125 - 131
Database
ISI
SICI code
0270-4137(20010501)47:2<125:PANSNP>2.0.ZU;2-3
Abstract
BACKGROUND. The database of human Expressed Sequence Tags (dbEST) provides a potential source for identification of tissue-specific genes. This databa se contains sequences that originate from cDNA libraries from particular tu mors, organs or cell typos. In this report, we have used the EST database t o identify PRAC, a novel gene specifically expressed in human Prostate, pro state cancer, Rectum And distal Colon. METHODS. Using a computer based analysis, a cluster of sequence homologous ESTs was identified which contained ESTs derived only from human prostate c DNA libraries. The tissue specificity was examined by multiple tissue RNA d ot blots and RT-PCR. The PRAC transcript and protein Mas identified using N orthern blot analysis, RACE-PCR, primer extension, and western blot. RESULTS. PRAC encode a 382 nucleotide RNA found in prostate, rectum, distal colon, and in three prostate cancer cell lines; LNCaP, PC-3 and DU145. Thi s transcript encodes a 6 kDa nuclear protein. The PRAC gene is located on c hromosome 17 at position 17q21, about 4 kbp downstream from the homeodomain Hoxb-13 gene. CONCLUSIONS. Our data proves that the EST database can be a useful tool for discovery of prostate-specific genes. The nuclear localization, identifica tion of potential phosphorylation sites, and possible cotranscription with the Hoxb-13 gene suggest that PRAC may have a regulatory role in the nucleu s. Prostate 47:125-131, 2001. (C) 2001 Wiley-Liss, Inc.