Effects of erysimin G on renal tubular function and 70-pS K+ channel activity of thick ascending limb

AIM: To study the effect of erysimin G (CH35H52O13) on the thick ascending limb (TAL) 70-pS K+ channel of rat kidney and its effect on diuresis. METHO DS: The patch-clamp cell-attached recording technique was used to record th e single potassium channel current, and the urine volume (UV) was measured by urethal intubation to determine the diuretic effect. RESULTS: Erysimin G can increase the urine volume and decrease the 70-pS potassium channel act ivity of TAL. CONCLUSION: Erysimin G has a diuretic effect and its inhibiti on on the activity of apical 70-pS potassium channel may be the mechanism o f its diuretic effect.