Metabolism of chiisanoside from Acanthopanax divaricatus var. albeofructusby human intestinal bacteria and its relation to some biological activities

The metabolic pathway of chiisanoside isolated from leaves of Acanthopanax divaricatus var. albeofructus (Araliaceae) by human intestinal bacteria and by the protein fraction of leaves of this plant were investigated, and the cytotoxic and anti-rotaviral activities of chiisanoside and its metabolite , chiisanogenin, were assayed. Chiisanogenin was produced as a main metabol ite, when chiisanoside were incubated for 15h with human intestinal bacteri a. This metabolic pathway proceeded more potently,with the protein fraction than with human intestinal bacteria. The irt vitro cytotoxicity of chiisan ogenin was superior to that of chiisanoside, H+/H+ ATPase was more potently inhibited by chiisanogenin than by chiisanoside, However, the anti-rotavir al activity of chiisanoside was more potent than that of chiisanogenin.