Quantitative two-color fluorescence cross-correlation spectroscopy in the analysis of polymerase chain reaction

We present results of an approach in which low-density labeled DNA itself p rovides an amplification of the cross-correlated fluorescent signal in the two-color cross- correlation function. Tetramethylrhodamine-4-dUTP and Cy5- dCTP are incorporated by polymerase chain reaction at multiple positions of the same 217 bp target DNA. We call this novel approach the 'two-color FCS signal amplification'. The signal amplification is an example for interact ions of two ligands with different colors at multiple positions of the same target.