Isolation, purification and characterization of secondary structure of antifreeze protein from Ammopiptanthus mongolicus

Authors
Fei, YB Wei, LB Gao, SQ Lu, MC Wang, BH Li, ZF Zhang, YM Shu, NH Jiang, Y Wang, WX
Citation
Yb. Fei et al., Isolation, purification and characterization of secondary structure of antifreeze protein from Ammopiptanthus mongolicus, CHIN SCI B, 46(6), 2001, pp. 495-498
Citations number
17
Categorie Soggetti
Multidisciplinary
Journal title
CHINESE SCIENCE BULLETIN
ISSN journal
10016538 → ACNP
Volume
46
Issue
6
Year of publication
2001
Pages
495 - 498
Database
ISI
SICI code
1001-6538(200103)46:6<495:IPACOS>2.0.ZU;2-X
Abstract
Antifreeze protein(AFP) from Ammopiptanthus mongolicus leaves was isolated and purified with DEAE cellulose 52, Sephacryl 300, heat treatment and prep arative isotachophoresis. The molecular weight of AFP was 50 ku by SDS-PAGE measurement. The average thermal hysteresis activity (THA) of AFP was 0.35 degreesC at 5 mg/mL using a differential scanning calorimeter (DSC). This AFP showed heated stability. From circular dichroism (CD) of AFP spectral d ata from 195-240 nm, a well-defined secondary structure of 11% alpha -helix , 34% antiparallel beta -sheet and 55% random coil for the plant AFP was de duced.