Determination of momordicoside A in bitter melon by high-performance liquid chromotography after solid-phase extraction

Momordicoside A has been determined by solid-phase extraction (SPE) on a En vi Carb cartridge (3 mt, 250 mg) then high-performance liquid chromatograph y (HPLC) on a Cis column (4.6 mm x 250 mm, 5 mum particle) with acetonitril e-methanol-50 mM potassium dihydrogen phosphate buffer, 25:20:60 (v/v), as mobile phase, at a flow rate of 0.8 mi min(-1), and UV detection at 208 nm. The analytical method was shown to be highly reproducible, with precision (as RSD) and accuracy las RMD < 10%, both intra-day and inter-day. Absolute recoveries were > 90%. The method was applied to the determination of momo rdicoside A in various tissues from different varieties of bitter melon fro m different producing areas.