Pulsatile activity and hatching of in vitro produced cow blastocysts: Effects of serum supplementation

Authors
van Heule, A van Langendonckt, A Donnay, I Dessy, E Massip, A
Citation
A. Van Heule et al., Pulsatile activity and hatching of in vitro produced cow blastocysts: Effects of serum supplementation, EUR J MORPH, 39(2), 2001, pp. 73-79
Citations number
49
Categorie Soggetti
Experimental Biology
Journal title
EUROPEAN JOURNAL OF MORPHOLOGY
ISSN journal
09243860 → ACNP
Volume
39
Issue
2
Year of publication
2001
Pages
73 - 79
Database
ISI
SICI code
0924-3860(200104)39:2<73:PAAHOI>2.0.ZU;2-5
Abstract
The effect of serum added to a modified SOF medium on pulsatile activity an d hatching of in vitro produced cow blastocysts was investigated by time-la pse cinematography. Embryos were generated from abattoir material and cultu red in mSOF without serum or with 10% FCS added at 42 h pi. Addition of ser um significantly increases pulsatile activity before zona rupture and reduc es the time of hatching. Pulsatile activity does not seem to be involved in the hatching process.