M. Sillem et al., Regulation of matrix metalloproteinases and their inhibitors in uterine endometrial cells of patients with and without endometriosis, EUR J OB GY, 95(2), 2001, pp. 167-174
Citations number
23
Categorie Soggetti
Reproductive Medicine
Journal title
EUROPEAN JOURNAL OF OBSTETRICS GYNECOLOGY AND REPRODUCTIVE BIOLOGY
Objective: To determine whether alterations in the secretion and regulation
of matrix metalloproteinases (MMPs) and their inhibitors are present in ut
erine endometrial cells from endometriosis patients.
Study Design: In an in vitro study, uterine endometrial cells from 19 regul
arly cycling women with and 32 without endometriosis were treated with diet
hyl stilbestrol, promegestone (R5020), interleukin-1 (IL-1) and tumor necro
sis factor a (TNF-alpha). Culture supernatants were assayed for MMPs 1, 2,
3, and 9, and for tissue inhibitors of MMP (TIMP-1 and TIMP-2) by ELISA.
Results: MMP-3 was secreted in high concentrations, moderate concentrations
were seen for MMP-1 and MMP-2, and very low concentrations for MMP-9. Subs
tantially more TIMP-1 than TIMP-2 was secreted. MMP-1 and MMP-3 were unifor
mly attenuated by R5020, while MMP-2 was not influenced by hormone treatmen
t. MMP-3 was upregulated by TNF-alpha in all samples while IL-1 only increa
sed secretion in cells from endometriosis patients.
Conclusion: The upregulation of MMP-3 by IL-1 may contribute to an increase
d invasiveness of uterine endometrial fragments in endometriosis patients.
(C) 2001 Elsevier Science Ireland Ltd. All rights reserved.