SIMULTANEOUS DETERMINATION OF 8 LIPID-PEROXIDATION DEGRADATION PRODUCTS IN URINE OF RATS TREATED WITH CARBON-TETRACHLORIDE USING GAS-CHROMATOGRAPHY WITH ELECTRON-CAPTURE DETECTION
Ll. Dezwart et al., SIMULTANEOUS DETERMINATION OF 8 LIPID-PEROXIDATION DEGRADATION PRODUCTS IN URINE OF RATS TREATED WITH CARBON-TETRACHLORIDE USING GAS-CHROMATOGRAPHY WITH ELECTRON-CAPTURE DETECTION, Journal of chromatography B. Biomedical sciences and applications, 694(2), 1997, pp. 277-287
Citations number
29
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
One of the major processes that occur as a result of radical-induced o
xidative stress is lipid peroxidation (LPO). Degradation of lipid pero
xides results in various products, including a variety of carbonyl com
pounds. In the present study eight different lipid degradation product
s, i.e., formaldehyde, acetaldehyde, acetone, propanal, butanal, penta
nal, hexanal and malondialdehyde were identified and measured simultan
eously and quantitatively in rat urine after derivatization with 0-(2,
3,4,5,6-pentafluorbenzyl)hydroxylamine hydrochloride, extraction with
heptane and using gas chromatography-electron-capture detection (GC-EC
D). The identity of the respective oximes in urine was confirmed by ga
s chromatography-negative ion chemical ionization mass spectrometry (G
C-NCI-MS). Simultaneously measured standard curves were linear for all
oxime-products and the detection limits were between 39.0+/-5.3 (n=9)
and 500+/-23 (n=9) fmol per mu l injected sample. Recoveries of all p
roducts from urine or water were 73.0+/-5.2% and higher. In urine of C
Cl4-treated rats an increase in all eight lipid degradation products i
n urine was found 24 h following exposure. ACON showed the most distin
ct increase, followed by PROPA, BUTA and MDA. It is concluded that the
rapid, selective and sensitive analytical method based on GC-ECD pres
ented here is well suited for routine measurement of eight different l
ipid degradation products. These products appear to be useful as non-i
nvasive biomarkers for in vivo oxidative stress induced in rats by CCl
4. (C) 1997 Elsevier Science B.V.