IMPROVED METHOD FOR THE MEASUREMENT OF GLUTAMATE AND ASPARTATE USING CAPILLARY ELECTROPHORESIS WITH LASER-INDUCED FLUORESCENCE DETECTION AND ITS APPLICATION TO BRAIN MICRODIALYSIS
La. Dawson et al., IMPROVED METHOD FOR THE MEASUREMENT OF GLUTAMATE AND ASPARTATE USING CAPILLARY ELECTROPHORESIS WITH LASER-INDUCED FLUORESCENCE DETECTION AND ITS APPLICATION TO BRAIN MICRODIALYSIS, Journal of chromatography B. Biomedical sciences and applications, 694(2), 1997, pp. 455-460
Citations number
7
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
We have previously published data on the analysis of glutamate in micr
odialysis samples using a commercially availble CE apparatus. Here we
demonstrate further improvements in the analysis of both glutamate and
aspartate from very small volume microdialysates. The limit of detect
ion of our system has been increased to 10(-9) M for both glutamate an
d aspartate. This permits microdialysis sampling time to be reduced to
2 min, thus improving the temporal resolution of microdialysis sampli
ng. Concurrently, migration time has also been reduced such that resol
ution of both amino acids can be achieved inside 2 min. This new analy
tical method has been applied to the measurement of the EAA from micro
dialysis samples from the dentate gyrus of the hippocampus. Extracellu
lar concentrations of both glutamate and aspartate increased to a maxi
mum of 5- and 4.5-fold of preinfusion values, respectively, during inf
usion of 100 mM K+ through the microdialysis probe. This is consistent
with the depolarization-evoked release of both amino acids from this
brain region. (C) 1997 Elsevier Science B.V.