IDENTIFICATION AND QUANTITATIVE-DETERMINATION OF URIC-ACID IN HUMAN URINE AND PLASMA BY CAPILLARY ELECTROPHORESIS WITH AMPEROMETRIC DETECTION

Citation
Dk. Xu et al., IDENTIFICATION AND QUANTITATIVE-DETERMINATION OF URIC-ACID IN HUMAN URINE AND PLASMA BY CAPILLARY ELECTROPHORESIS WITH AMPEROMETRIC DETECTION, Journal of chromatography B. Biomedical sciences and applications, 694(2), 1997, pp. 461-466
Citations number
12
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
ISSN journal
13872273
Volume
694
Issue
2
Year of publication
1997
Pages
461 - 466
Database
ISI
SICI code
0378-4347(1997)694:2<461:IAQOUI>2.0.ZU;2-T
Abstract
Application of capillary zone electrophoresis with electrochemical det ection to the identification and quantitative determination of uric ac id in human urine as well as plasma is described. This work was carrie d out in a 30 cmx25 mu m I.D. fused-silica capillary with tricine buff er and a carbon fiber bundle was employed as a working electrode, the working voltage in amperometric detection was set at +0.80 V (vs. SCE) . The sample constituent is identified by stopped how-linear sweep vol tammetry. Under optimal conditions, a lower detection limit of 0.48 fm ol was obtained for uric acid. (C) 1997 Elsevier Science B.V.