IDENTIFICATION AND QUANTITATIVE-DETERMINATION OF URIC-ACID IN HUMAN URINE AND PLASMA BY CAPILLARY ELECTROPHORESIS WITH AMPEROMETRIC DETECTION

Application of capillary zone electrophoresis with electrochemical det ection to the identification and quantitative determination of uric ac id in human urine as well as plasma is described. This work was carrie d out in a 30 cmx25 mu m I.D. fused-silica capillary with tricine buff er and a carbon fiber bundle was employed as a working electrode, the working voltage in amperometric detection was set at +0.80 V (vs. SCE) . The sample constituent is identified by stopped how-linear sweep vol tammetry. Under optimal conditions, a lower detection limit of 0.48 fm ol was obtained for uric acid. (C) 1997 Elsevier Science B.V.