Less is more - length reduction of STR amplicons using redesigned primers

Authors
Wiegand, P Kleiber, M
Citation
P. Wiegand et M. Kleiber, Less is more - length reduction of STR amplicons using redesigned primers, INT J LEGAL, 114(4-5), 2001, pp. 285-287
Citations number
13
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology
Journal title
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
ISSN journal
09379827 → ACNP
Volume
114
Issue
4-5
Year of publication
2001
Pages
285 - 287
Database
ISI
SICI code
0937-9827(200104)114:4-5<285:LIM-LR>2.0.ZU;2-U
Abstract
PCR primers closely flanking the repeat region were redesigned to reduce th e amplicon length of the selected STRs down to approximately 100 bp for the shorter alleles (loci HumTH01, D10S2325, DYS19 and DYS391). Highly degrade d DNA (e.g. formalin-fixed tissue) and very low amounts of DNA could be mor e successfully typed using the new redesigned primers compared to the estab lished sequences generating longer amplicons.