Aims: To investigate the effect of whey protein concentrate (WPC) enriched
in anti-Helicobacter pylori antibodies on growth of the organism in vitro.
Methods and Results: A WPC rich in H. pylori-specific antibodies was produc
ed by immunizing lactating cows against H. pylori and processing pooled bul
k milk samples into whey powder. The antibodies bound several proteins with
in the bacterial homogenate and were active at pH 5. In a complement-depend
ent reaction, the immune WPC was highly bactericidal against four H. pylori
strains tested in vitro.
Conclusions: WPC produced with milk from H. pylori-immunized cows contains
antibodies that are active at the pH of the stomach, and bactericidal again
st H. pylori in vitro, via the classical complement pathway.
Significance and Impact of the Study: This study has demonstrated the poten
tial for use of WPC in the prevention/treatment of H. pylori infections.