Localization of neuronal growth-associated, microtubule-destabilizing factor SCG10 in brain-derived raft membrane microdomains

Citation
S. Maekawa et al., Localization of neuronal growth-associated, microtubule-destabilizing factor SCG10 in brain-derived raft membrane microdomains, J BIOCHEM, 129(5), 2001, pp. 691-697
Citations number
56
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF BIOCHEMISTRY
ISSN journal
0021924X → ACNP
Volume
129
Issue
5
Year of publication
2001
Pages
691 - 697
Database
ISI
SICI code
0021-924X(200105)129:5<691:LONGMF>2.0.ZU;2-E
Abstract
Raft is a mobile membrane subdomain enriched in sphingolipid and cholestero l and also various signaling molecules. Previous observation suggested that brain-derived rafts contain tubulin but that rafts of non-neural origin do not. We hypothesized that SCG10, one of the neuronal growth-associated pro teins (nGAPs), might be a neuron-specific molecule that anchors tubulin. to neuronal rafts, and we explored biochemically its subcellular localization , interaction with tubulin, and effects on microtubule dynamics. In postnat al rat brain extracts, SCG10 was recovered mostly in membrane-associated fr actions, and at least half was included in the raft fraction that was also enriched in GAP-43 and NAP-22. SCG10-enriched brain rafts also contained tu bulin, and chemical cross-linking experiments revealed that SCG10 was close ly associated with tubulin, In addition, SCG10 was able to inhibit polymeri zation of tubulin, These results indicate that SCG10 is a component of neur onal rafts as are other nGAPs, and suggest that SCG10 may be involved in si gnaling events in membranes for cytoskeletal reorganization around neuronal rafts.