Kv beta subunits increase expression of Kv4.3 channels by interacting withtheir C termini

Auxiliary Kv beta subunits form complexes with Kv1 family voltage-gated Kchannels by binding to a part of the N terminus of channel polypeptide. Thi s association influences expression and gating of these channels. Here we s how that Kv4.3 proteins are associated with Kv beta2 subunits in the brain. Expression of Kv beta1 or Kv beta2 subunits does not affect Kv4.3 channel gating but increases current density and protein expression. The increase i n Kv4.3 protein is larger at longer times after transfection, suggesting th at Kv beta -associated channel proteins are more stable than those without the auxiliary subunits. This association between Kv4.3 and Kv beta subunits requires the C terminus but not the N terminus of the channel polypeptide. Thus, Kv beta subunits utilize diverse molecular interactions to stimulate the expression of Kv channels from different families.