L. Carpenter et al., Protein kinase C delta activation by interleukin-1 beta stabilizes inducible nitric-oxide synthase mRNA in pancreatic beta-cells, J BIOL CHEM, 276(7), 2001, pp. 5368-5374
Exposure of pancreatic islets to cytokines such as interleukin (IL)-1 beta
induces a variety of proinflammatory genes including type II nitric-oxide s
ynthase (MOS) which produces nitric oxide (NO). NO is thought to be a major
cause of islet beta -cell dysfunction and apoptotic beta -cell death, whic
h results in type I diabetes. Since protein kinase C (PKC) mediates some of
the actions of cytokines in other cell types, our aim was to assess the ro
le of PKC in IL-1 beta -induced iNOS expression in pancreatic beta -cells.
PKC delta, but not PKC alpha, was specifically activated in the rat INS-1 b
eta -cell line by IL-1 beta as assessed by membrane translocation. Moreover
, MOS expression and NO production were significantly attenuated by the PKC
delta specific inhibitor rottlerin and overexpression of a PKC delta kinas
e-dead mutant protein. Conversely, overexpression of PKC delta wild type pr
otein significantly potentiated this response. These results were confirmed
at the mRNA level by reverse transcriptase-polymerase chain reaction. Howe
ver, a role at the level of transcriptional regulation appeared unlikely, s
ince PKC delta was not required for the activation of NF-kappaB, activating
protein 1, and activating transcription factor 2 signaling pathways in res
ponse to IL-1 beta. There was, however, a significant increase in MOS mRNA
stability mediated by PKC delta wild type, while PKC delta kinase-dead acte
d reciprocally, reducing MOS mRNA stability. The results indicate that, in
addition to transcriptional activation, mRNA stabilization is a key compone
nt of the mechanism by which IL-1 beta stimulates MOS expression in beta -c
ells and that PKC delta plays an essential role in this process. PKC delta
activation may therefore have significant consequences with regard to cellu
lar function and viability when beta -cells are exposed to IL-1 beta and po
tentially other cytokines.