Application of capillary electrochromatography using macroporous polyacrylamide columns for the analysis of lignans from seeds of Schisandra chinensis

Capillary electrochromatography (CBC) using polymer-based monolithic statio nary phase has been developed as a promising method for the determination o f lignans of Schisandra chinensis. The columns were prepared by in situ cop olymerisation of acrylamide, N,N'-methylenebisacrylamide, vinylsulfonic aci d and lauryl acrylate in presence of poly(ethylene glycol) as a porogenic a gent. The columns [33 cm (24.5 cm effective length)X75 mum I.D.] were succe ssfully used to analyse and quantify the major lignans in extract of the se eds of Schisandra chinensis. Good separations were achieved in less than 35 min. The calibration graphs were Linear in the range 0.025-1.0 mg/ml of gi ven lignan with correlation coefficients between 0.9951 and 0.9996. The int er-day reproducibility of the peak area were below 3.9% and the inter-day r eproducibility of the migration time were below 4.2%. The results of quanti tative CEC analyses were compared with those obtained by reversed-phase HPL C, the levels of schizandrin, gomisin A, gomisin N and wuweizisu C determin ed by CEC were in a good agreement with those determined by HPLC. (C) 2001 Elsevier Science B.V. All rights reserved.