Chiral selective separation of tocainide by capillary electrophoresis using various cyclodextrin derivatives

Chiral separation of tocainide, an antiarrythmic agent, was solved using th e capillary electrophoresis method. Fifteen types of cyclodextrins were tri ed as chiral selective buffer additives, and baseline separation was achiev ed with five of them. The variables that were studied comprise, type and co ncentration of the chiral selectors, pH of the buffers, and the organic mod ifiers. The best resolution, an R-s value of 5.2, was achieved using 7.5 mM gamma -cyclodextrin phosphate at pH 6.8. Migration reversal of enantiomers was observed when changing the type of cyclodextrins, Not only the tocaini de but also its by-product were enantiomericaly separated. (C) 2001 John Wi ley & Sons, Inc.