Select soft tissues in clubfeet are contracted, resulting in stiffness. The
se contracted tissues share ultrastructural characteristics with palmar fib
romatosis (Dupuytren contracture), in which the growth factors transforming
growth factor-beta (TGF-beta) and platelet-derived growth factor (PDGF) ar
e expressed and play a role in regulating cell behavior. More contracted ti
ssue (medial side of the foot) and less contracted tissue (lateral side of
the foot) from 20 clubfeet were studied using reverse transcription-polymer
ase chain reaction and western analysis for expression of TGF-beta and PDGF
(along with collagen type I and type III). Cell cultures were established
from the more contracted tissues to determine the effect of blockade of the
se factors with neutralizing antibodies on proliferation, chemotaxis. and c
ollagen expression. Both growth factors were expressed at higher levels by
the contracted tissues, and blockade led to decreased collagen expression,
proliferation, and chemotaxis, Growth factor blockade has the potential to
change the behavior of these cells in a way that would lessen the severity
of the contractures. perhaps improving the outcome of clubfoot treatment.