The surface-enhanced Raman scattering (SERS) spectra of polydrin of Trabala
vishnou nuclear polyhedrosis virus (sample 1) and polydrin of Dendrolimus
punctatus nuclear polyhedrosis virus (sample 2) adsorbed in silver hydrosol
were investigated. The features in the adsorption mode, enhancement charac
ter and mechanism of the SERS spectra of these two samples were compared an
d are discussed. It was found that the molecules of sample 1 adsorbed on th
e silver surface through carboxyl and amino groups, and the molecules of sa
mple 2 through the sulfur atom and also carboxyl and amino groups. The chem
isorption of the molecules of both samples and their short-range character
indicate that the molecular mechanism is the primary enhancement in the SER
S spectra of molecules of these h ro insect viruses. Copyright (C) 2001 Joh
n Wiley & Sons, Ltd.