Structural characterization of flavonoid glycosides by collisionally activated dissociation of metal complexes

Collisionally activated dissociation is used for structural characterizatio n of a series of flavonoid glycosides. Dissociation of transition metal/fla vonoid binary complexes of the type [M-II(L - (+))](+) and transition metal /2,2'-bipyridine/flavonoid ternary complexes of the type [M-II(L - H+)bpy]( +) give fragmentation patterns that are complementary and more diagnostic t han those of the protonated, deprotonated, or sodium-cationized flavonoids. Analysis of fragmentation patterns of the [M-II(L - H+)bpy](+) complexes p ermits determination of the disaccharide as a rutinose or neohesperidose an d the relative placement of the disaccharide (i.e., 3 vs. 7 positions). (J Am Soc Mass Spectrom 2001, 12, 537-549) (C) 2001 American Society for Mass Spectrometry.