The quality of a sine vinegar is determined by the raw wine substrate and t
he acetification process employed in its production. Attempts to characteri
se vinegars have been based on these two features, along with variables suc
h as total extract, glycerol, organic acids, volatile compounds and phenoli
c composition. When the final products are analysed, it is difficult to eva
luate to what extent quality differences are due to the raw material or to
differences in production methods, so it is necessary to determine the infl
uence of each feature separately. The present work focuses on monitoring ph
ysicochemical changes during the acetification of sherry wine by submerged
culture. ANOVA showed significant differences for ethanol, acetic and lacti
c acids and some volatile compounds (methanol, l-propanol, 2-methyl-1-propa
nol, 2-methyl-1-butanol, 3-methyl-1-butanol, acetoin, acetaldehyde, ethyl a
cetate and ethyl lactate). However, no statistical changes were found for p
henolic compounds during acetification. The phenolic composition of the fin
al product was determined by the substrate employed. In addition, the influ
ence of cycle duration on the chemical composition was studied; the only co
mpound affected by this factor was 2-methyl-1-butanol. (C) 2001 Society of
Chemical Industry.