M. Seguchi et al., EFFECT OF A WHEAT-PROTEIN ON PH-DEPENDENT WATER-BINDING CAPACITY AND VISCOSITY OF WHEAT TAILINGS FRACTIONS, Cereal chemistry, 74(4), 1997, pp. 384-388
Wheat flour was fractionated with acetic acid using a mortar and pestl
e method or a blender method. Higher pH-dependent water-binding capaci
ty (WBC) and viscosity were obtained only in the tailings fraction. Th
e higher pH-dependent WBC was rather stable at 5-37 degrees C, however
it decreased with salt addition. Pepsin or bromelain treatment stoppe
d the pH-dependent changes in WBC and viscosity, which suggests that t
his characteristic of the tailings fraction is due to the presence of
proteins. HPLC indicated that the M-r of the proteins associated with
high WBC at low pH was >200 kDa.