Intracellular trafficking of retroviral RNAs is a potential mechanism to ta
rget viral gene expression to specific regions of infected cells. Here we s
how that the human immunodeficiency virus type 1 (HIV-1) genome contains tw
o sequences similar to the hnRNP A2 response element (A2RE), a cis-acting R
NA trafficking sequence that binds to the trans-acting trafficking factor,
hnRNP A2, and mediates a specific RNA trafficking pathway characterized ext
ensively in oligodendrocytes. The two HIV-1 sequences, designated A2RE-1, w
ithin the major homology region of the gag gene, and A2RE-2, in a region of
overlap between the vpr and tat genes, both bind to hnRNP A2 in vitro and
are necessary and sufficient for RNA transport in oligodendrocytes in vivo.
A single base change (A8G) in either sequence reduces hnRNP A2 binding and
, in the case of A2RE-2, inhibits RNA transport. A2RE-mediated RNA transpor
t is microtubule and hnRNP A2 dependent. Differentially labelled gag and vp
r RNAs, containing A2RE-1 and A2RE-2, respectively, coassemble into the sam
e RNA trafficking granules and are cotransported to the periphery of the ce
ll. tat RNA, although it contains A2RE-2, is not transported as efficiently
as vpr RNA. An A2RE/hnRNP A2-mediated trafficking pathway for HIV RNA is p
roposed, and the role of RNA trafficking in targeting HIV gene expression i
s discussed.