MODULATION OF POTASSIUM-EVOKED [H-3] DOPAMINE RELEASE FROM RAT STRIATAL SLICES BY VOLTAGE-ACTIVATED CALCIUM-CHANNEL LIGANDS - EFFECTS OF OMEGA-CONOTOXIN-MVIIC
D. Dobrev et K. Andreas, MODULATION OF POTASSIUM-EVOKED [H-3] DOPAMINE RELEASE FROM RAT STRIATAL SLICES BY VOLTAGE-ACTIVATED CALCIUM-CHANNEL LIGANDS - EFFECTS OF OMEGA-CONOTOXIN-MVIIC, Neurochemical research, 22(9), 1997, pp. 1085-1093
We examined the involvement of voltage-activated Ca2+ channels (VACCs)
on K+(50 mM)-evoked [H-3]dopamine ([H-3]DA) release from superfused r
at striatal slices. Neither nifedipine nor nitrendipine modified K+-ev
oked [SH]DA release, indicating that L-type VACCs are not involved. K-evoked [H-3]DA release was partially inhibited by omega-CTx-GVIA and
omega-Aga-IVA, and was abolished by 3 mu M omega-CTx-MVIIC (IC50 simil
ar to 128 nM), suggesting the involvement of N-, P-, or Q-type VACCs,
respectively. Moreover, even subnanomolar concentrations of omega-CTx-
MVIIC (0.1-0.5 nM) inhibited K+-evoked [H-3]DA release by similar to 2
5%, suggesting the possible involvement of a still not classified (per
haps O-type?) Ca2+ channel subtype. The effects of omega-CTx-MVIIC (10
-100 nM) and omega-CTx-GVIA (1 mu M) were additive, suggesting that lo
w nanomolar concentrations of omega-CTx-MVIIC does not interact with N
-type VACCs. In conclusion, the K+-evoked [H-3]DA release from rat str
iatal slices is mediated by entry of Ca2+ through omega-CTx-GVIA sensi
tive (N-type) as well as through omega-Aga-IVA (P-type) and omega-CTx-
MVIIC (probably Q-type) sensitive VACCs.