MULTI-RESIDUE SCREENING AND CONFIRMATORY ANALYSIS OF ANABOLIC-STEROIDS IN URINE BY GAS-CHROMATOGRAPHY COUPLED WITH TANDEM MASS-SPECTROMETRY

The diversity of substances used illegally as growth promoters in meat production requires the development of multi-analyte methods of analy sis involving a sample pretreatment step that is as rapid and as easy as possible, followed by a specific and sensitive determination of sev eral residues within the same run. A general strategy for the screenin g and confirmatory analysis of fifteen artificial anabolic compounds i n urine samples is described. It is based on solid-phase extraction on C-18 Empore discs and amino-bonded columns followed, after derivatiza tion (trimethylsilyl or methyloxime-trimethylsilyl derivatives), by ga s chromatography coupled with collisionally activated dissociation tan dem mass spectrometry.