Analysis of mustard trypsin inhibitor-2 gene expression in response to developmental or environmental induction

Transcription analysis of a mustard (Sinapis ulba L.) serine proteinase inh ibitor gene revealed identical 5 ' termini of mRNAs synthesized during seed maturation and chemical or wounding induction. Polyadenylation of mRNAs on multiple or single sites differentiated gene expression, increasing the av ailability of stable mRNAs during seed maturation compared with chemical an d wounding induction. Expression of the P-glucuronidase (GUS)-encoding regi on of the UidA reporter gene. detected under the control of deleted segment s of the region flanking on the 5 ' side the mti-2 gene. identified a stret ch of about 520 bp essential for gene expression. The presence in this regi on of two ABRE motifs is relevant for plant response to gene induction. Exp ression of GUS was detectable under different induction stimuli in several organs such as seedlings and leaves and was active to varying extents in th e vascular tissues and meristem.