Selenomethionine incorporation in Saccharomyces cerevisiae RNA polymerase II

Authors
Bushnell, DA Cramer, P Kornberg, RD
Citation
Da. Bushnell et al., Selenomethionine incorporation in Saccharomyces cerevisiae RNA polymerase II, STRUCTURE, 9(1), 2001, pp. R11-R14
Citations number
13
Categorie Soggetti
Biochemistry & Biophysics
Journal title
STRUCTURE
ISSN journal
09692126 → ACNP
Volume
9
Issue
1
Year of publication
2001
Pages
R11 - R14
Database
ISI
SICI code
0969-2126(20010110)9:1<R11:SIISCR>2.0.ZU;2-A
Abstract
A protocol for the incorporation of SeMet into yeast proteins is described. Incorporation at a level of about 50% suffices for the location of Se site s in an anomalous difference Fourier map of the 0.5 MDa yeast RNA polymeras e II. This shows the utility of the approach as an aid in the model-buildin g of large protein complexes.