Identification of inter simple sequence repeat (ISSR) markers associated with seed size in wheat

The feasibility of identifying inter-simple sequence repeat markers associa ted with seed weight in hexaploid wheat was tested using 113 recombinant in bred lines developed by the single-seed descent method, from a cross betwee n Rye selection111, an Indian genetic stock obtained through the introgress ion of genes for bold seed size from rye, and Chinese Spring having small s eed size. Three markers were associated with low seed size with gene effect s of 14.8%, 9.5%, and 6%, while four markers with contributions of 8%, 4.66 %, 2.92% and 2.61% were found to be linked to high seed size, together cont ributing 31% of the phenotypic variance in seed size. Nulli-tetrasomic and di-telosomic analysis revealed the presence of three low seed size QTL-asso ciated markers on three chromosomes, 6BL, 2DL, and 1DS respectively. This s tudy clearly demonstrates that ISSRs are highly useful for finding markers associated with major and minor genes controlling agronomically important t raits in wheat.