A piezoelectric biomimetic sensor for aminopyrine with a molecularly imprinted polymer coating

A molecularly imprinted polymer for aminopyrine was synthesized using metha crylic acid as functional monomer. The polymer was employed as the recognit ion element of a piezoelectric bulk acoustic wave biomimetic sensor for ami nopyrine. Influencing factors were investigated in detail and optimized. Th is sensor exhibited high selectivity and sensitivity to aminopyrine. The re sponse range of the sensor was between 5.0 x 10(-8) and 1.0 x 10(-4) M with a detection limit of 2.5 x 10(-8) M in the aqueous system. Scatchard analy sis with UV spectrophotometry showed that the same class of binding sites w as formed in the molecularly imprinted polymer in the studied concentration range, and the dissociation constant and the apparent maximum number of th ese binding sites were estimated to be 2.29 mM and 165.0 mu mol g(-1) dry p olymer, respectively. Impedance analysis was employed to verify the imprint ing effect and lack of variation in the viscoelasticity of the polymer coat ing during detection.