A micro-polarimeter with a 40 nL probe volume was configured so that it is
compatible with capillary-scale flowing stream analysis. The optical config
uration consists of two polarizing optics, a capillary, a laser source and
a photodetector which is very simple to configure with low cost components.
This unique polarimeter is based upon the interaction of a linearly polari
zed laser beam and a capillary tube, in this case one with an inner diamete
r of 250 mum. Side illumination of the tube results in a 360 degrees fan of
scattered light, which contains a set of interference fringes that change
in response to optically active solutes. Solutes that exhibit optical activ
ity are quantifiable and are detected by analyzing the polarization state o
f the backscattered light. The ability of the instrument to make extremely
sensitive optical activity measurements in flowing streams is shown by the
determination of (R)-mandelic acid, with a detection limit of 66 x 10(-6) M
(507 x 10(-12) g), and the non-optically active control, glycerol. Additio
nally, the detector was configured to minimize refractive index perturbatio
ns.