Isolation of Magnetospirillum magneticum AMB-1 mutants defective in bacterial magnetic particle synthesis by transposon mutagenesis

Nonmagnetic mutants of Magnetospirillum magneticum AMB-1 were recovered fol lowing mini-Tn5 transposon mutagenesis. Transconjugants with kanamycin resi stance were obtained at a frequency of 2.7 x 10(-7) per recipient. Of 3327 transconjugants, 62 were defective for bacterial magnetic particle (BMP) sy nthesis. The frequency of independent transposition events for nonmagnetic mutants was about 1.4% in transconjugants. Further analysis of DNA sequence s flanking transposon by inverted polymerase chain reaction allowed isolati on of at least 10 genes or DNA sequences involved in BMP synthesis in M. ma gneticum AMB-1.