Pilot plant trials were conducted in a corn wet mill with a 7000-L membrane
recycle bioreactor (MRB) that integrated ceramic microfiltration membranes
in a semi-closed loop configuration with a stirred-tank reactor. Residence
times of 7.5-10 h with ethanol outputs of 10-11.5% (v/v) were obtained whe
n the cell concentration was 60-100 g/L dry wt of yeast, equivalent to abou
t 10(9)-10(10) cells/mL. The performance of the membrane was dependent on t
he startup mode and pressure management techniques. A steady flux of 70 L/(
m(2.)h) could be maintained for several days before cleaning was necessary.
The benefits of the MRB include better productivity; a clear product strea
m containing no particulates or yeast cells, which should improve subsequen
t stripping and distillation operations; and substantially reduced stillage
handling. The capital cost of the MRB is $21-$34/(m(3.)pr) ($0.08-$0.13/ [
gal(.)yr]) of ethanol capacity. Operating cost, including depreciation, ene
rgy, membrane replacement, maintenance, labor, and cleaning, is $4.5-9/m(3)
($0.017-$0.034/gal) of ethanol.