Kinetic conditions were established for the depolymerization of cassava sta
rch for the production of maltodextrins and glucose syrups. Thin-layer chro
matography and high-performance liquid chromatography analyses corroborated
that the proper H,PO, strength and thermopressurization range (e.g., 142-1
70 degreesC; 2.8-6.8 atm) can be successfully explored for such hydrolytic
purposes of native starch granules. Because phosphoric acid can be advantag
eously maintained in the hydrolysate and generates, after controlled neutra
lization with ammonia, the strategic nutrient triplet for industrial fermen
tations (C, P, N), this pretreatment strategy can be easily recognized as a
recommended technology for hydrolysis and upgrading of starch and other pl
ant polysaccharides. Compared to the classic catalysts, the mandatory desal
ting step (chloride removal by expensive anion-exchange resin or sulfate pr
ecipitation as the calcium-insoluble salt) can be avoided. Furthermore, pro
perly diluted phosphoric acid is well known as an allowable additive in sev
eral popular soft drinks such as colas since its acidic feeling in the mout
h is compatible and synergistic with both natural and artificial sweeteners
. Glycosyrups from phosphorolyzed cassava starch have also been upgraded to
high-value single-cell protein such as the pigmented yeast biomass of Xant
hophyllomyces dendrorhous (Phaffia rhodozyma), whose astaxanthin (wiketo-di
hydroxy-beta -carotene) content may reach 0.5-1.0 mg/g of dry yeast call. T
his can he used as an ideal complement for animal feeding as well as a natu
ral staining for both fish farming (meat) and poultry (eggs).