Immunohistochemical detection of a very high density lipoprotein (VHDL) inovarian follicles of Triatoma infestans

Citation
Ms. Gonzalez et al., Immunohistochemical detection of a very high density lipoprotein (VHDL) inovarian follicles of Triatoma infestans, BIOCELL, 25(1), 2001, pp. 23-27
Citations number
31
Categorie Soggetti
Cell & Developmental Biology
Journal title
BIOCELL
ISSN journal
03279545 → ACNP
Volume
25
Issue
1
Year of publication
2001
Pages
23 - 27
Database
ISI
SICI code
0327-9545(200104)25:1<23:IDOAVH>2.0.ZU;2-U
Abstract
The ability of Triatoma infestans ovarian follicles to synthesize a very hi gh-density lipoprotein (VHDL) has been examined by immunohistochemical meth ods. This kind of lipoprotein can be envisaged as a storage hexameric prote in present in the hemolymph of some insect species. VHDL immunoreactivity i s observed in oocytes at different stages of maturation. The antigen is pre sent in the oocyte cytoplasm as well as in the follicular epithelial cells. The immunopositive reaction in the apical surface of follicle cells sugges ts both a VHDL synthesis and a secretion process. Furthermore, VHDL seems t o be stored into oocyte in yolk granules. On the contrary, no immunopositiv e reaction is observed in the intracellular spaces between follicle cells, suggesting that VHDL is not incorporated from hemolymph into the oocyte.