Binding of dimeric aminoglycosides to the HIV-1 Rev responsive element (RRE) RNA construct

Through a series of elegant fluorescence measurements: particularly through stopped-flow kinetic measurements, it was recently demonstrated that amino glycoside antibiotics are able to bind to the HIV-1 Rev responsive element (RRE) RNA construct in more than a 1:1 stoichiometry (Lacourciere, K. A.; S tivers, J. T.; Marine; J. P. Biochemistry 2000, 9, 5630). Here, we present the binding study results of dimeric neomycin Ligands through fluorescence anisotropy studies, to the HIV-I RRE RNA construct. The dimeric neomycin mo lecules are observed to be able to bind the HIV-1 RRE RNA construct approxi mately 17-fold higher when compared to the monomeric neomycin, lending evid ence that there are indeed two or more neomycin binding sites within the HI V-1 RRE construct. (C) 2001 Elsevier Science Ltd. All rights reserved.